Social determinants of violence against adolescent married women and how the women respond to it: A qualitative study in the Kurdish areas of Iran.

OBJECTIVE: It is crucial to identify the factors that influence violence against women, one of Iran's most significant health and social issues. Thus, the purpose of this research is to explore the social determinants of violence against adolescent married women and how the women respond to it in the Kurdish areas of Iran via a qualitative approach. METHOD: The present study was conducted with a qualitative approach and the conventional content analysis method among 33 married adolescent women who experienced violence and key informants in the Kurdish areas of Iran. Targeted and snowball sampling were used to reach the participants, and semistructured interviews were used to collect data. In order to improve the quality of the research results, Guba and Lincoln criteria were observed. RESULTS: Four categories, 18 subcategories, and 195 codes were obtained from the data analysis: (a) sociocultural factors (beliefs dictated by cultural norms, fatalism, incomplete social support, and weakness of the law), (b) family factors (the prevalence of silence in the face of violence, the existence of a model of violence and aggression in families, and inappropriate conditions of the husband), (c) individual factors (strong dependence on the husband, unstable position, low self-confidence, not having enough sexual skills, not having enough skills in interpersonal relationships, and too much insistence on continuing married life), and (d) reactions from the victims (silence and concealment, seeking support, tolerance, confrontation, and revenge). CONCLUSIONS: Violence against adolescent married women is influenced by various sociocultural, family, and individual factors, and women react differently to the violence of their husbands, the most common of which is silence and concealment. Therefore, prevention of this phenomenon requires individual, family, social, and cultural interventions. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Maternal psychological stress during pregnancy and newborn telomere length: a systematic review and meta-analysis.

INTRODUCTION: Telomeres protect the ends of chromosomes, and shorter leukocyte telomeres are associated with major group diseases. Maternal psychological stress may be related to the shortening of telomeres in infants. This systematic review and meta-analysis set out to consolidate the varying effect sizes found in studies of maternal psychological stress and telomere length (TL) in newborns and identify moderators of the relationship between stress during pregnancy and newborn TL. METHODS: Our systematic review was registered in Prospero. Six databases (PubMed, Scopus, Embase, PsycINFO, Web of Science, and CINAHL Complete) were searched for records in English from inception to February 10, 2023. Observational studies were included that measured the relationship of psychological stress of the mother during pregnancy on the TL of the newborn. The Newcastle-Ottawa quality assessment scale was used to assess the quality of the included studies. A random-effect model was selected. Statistical analysis performed by Stata software version 17. RESULTS: Eight studies were included for qualitative and four for quantitative analysis. There was an inverse statistically significant relationship between maternal stress and newborn TL; A one score increase in maternal psychological stress resulted in a 0.04 decrease in the TL of the newborn (B = -0.04, 95% CI = [-0.08, 0.00], p = 0.05). Selectivity analysis showed that the pooled effect size was sensitive to one study; After removing this study, the pooled effect size remained significant (B = -0.06, 95% CI = [-0. 10, -0.02], p < 0.001). CONCLUSION: Physiological and environmental factors can significantly affect the TL of newborns. Our results support a significant impact of maternal psychological stress on the TL of a newborn. This association demonstrates the significance of stress in influencing the telomere length, which can be a contributing factor in the infant's future. Therefore, recognizing this association is crucial for understanding and addressing potential health risks and necessitates the need for additional future studies to validate our findings.

Induction of Homosubtypic and Heterosubtypic Immunity to Influenza Viruses Using a Conserved Internal and External Proteins.

The immunogenicity and protective properties of the designed recombinant fusion peptide of 3M2e and truncated nucleoprotein (trNP), originating from Influenza A virus, were investigated in the BALB/c mice model in comparison with the Mix protein (3M2e + trNP). The results were evaluated by antibody response, cytokine production, lymphocyte proliferation assay, and mortality rate after challenge with homologous (H1N1) and heterologous (H3N2) influenza viruses in BALB/c mice. The animals that received the chimer protein with or without adjuvant had more specific antibody responses and elicited memory CD4 T cells, and cytokines of Th1 and Th2 cells compared to the Mix protein. Moreover, the Mix protein, like the recombinant chimer protein, provided equal and effective protection against both homologous and heterologous challenges in mice. Nevertheless, the chimer protein demonstrated superior immune protection compared to the Mix protein. The percentage of survived animals in the adjuvanted protein group (78.4%) was less than the non-adjuvanted one (85.7%). However, the Mix protein plus Alum could induce protective immunity in only 57.1% and 42.8% of homologous and heterologous virus-challenged mice, respectively. Regarding the sufficient immunogenicity and protectivity of the chimer protein construct against influenza viruses, the findings of the study suggest that the chimer protein without a requirement of adjuvant can be used as an adequate vaccine formulation to protect against a broad spectrum of influenza viruses.

Fracture Behavior of AA7075-AA6061 and AA7075-Cu Friction-Stir Welded Joints Containing Blunt V-Notches under Opening-Mode Loading.

The purpose of this study is to predict the load-bearing capacity (LBC) of fracture specimens containing V-notched friction-stir welded (FSWed) joints of AA7075-Cu and AA7075-AA6061 materials and subjected to mode I loading conditions. Due to the resulting elastic-plastic behavior and the corresponding development of significant plastic deformations, the fracture analysis of the FSWed alloys requires elastic-plastic fracture criteria, which are complex and time-consuming. Thus, in this study, the equivalent material concept (EMC) is applied, equating the actual AA7075-AA6061 and AA7075-Cu materials to equivalent virtual brittle materials. Then, two brittle fracture criteria, the maximum tangential stress (MTS) and mean stress (MS), are utilized to estimate the LBC of the V-notched FSWed parts. The comparison between the experimental results and the theoretical predictions reveals that both fracture criteria, in combination with EMC, can accurately predict the LBC in the analyzed components.

The Co-Use of Stromal Vascular Fraction and Bone Marrow Concentrate for Tendon Healing.

OBJECTIVE: The Achilles tendon is the most frequently injured tendon in the human body, despite being the strongest. Many conventional treatments including medication, surgical interventions, and physical therapy are available, however, the desired results are often not achieved. Stromal vascular fraction (SVF) and bone marrow concentrate (BMC) are two additional cellular treatment options. The purpose of this study is to evaluate the effect of SVF and BMC, used as a combination, for the treatment of Achilles tendon injuries. METHODS: Five male New Zealand rabbits were used for each of the 6 study groups. A 3-mm of SVF and BMC were injected on the Achilles tendons at certain ratios. The histological results were classified by the Movin grading system for tendon healing. The collagen type-I and type-III structures in the tendons were examined by immunohistochemical evaluation. The expressions of tendon-specific genes were also examined by using the RT-PCR method to analyze tendon healing. RESULTS: Histological and immunohistochemical evaluation indicated that tendons receiving the SVF and BMAC mixture performed better than control and individual groups (p < 0.05). Moreover, RT-PCR evaluation showed that mixture-receiving groups were the closest similar to the uninjured group (p < 0.05). CONCLUSION: The combined use of BMC and SVF improved Achilles tendon healing when compared to the individual use of each mixture.

The Effects of Glycerophospholipid Nanomicelles on the Cryotolerance of Frozen-Thawed Rooster Sperm.

Semen banking is an efficient method of artificial insemination for commercial breeders. However, the cryopreservation process induces severe damages to plasma membranes, which leads to reduced fertility potential of thawed sperm. The replacement of membrane lipids with oxidized membrane lipids repairs the cell membrane and improves its stability. The aim of this study was to investigate the effects of glycerophospholipid (GPL) nanomicelles on the cryosurvival of thawed rooster semen. Semen samples were collected from six 29-week Ross broiler breeder roosters, then mixed and divided into five equal parts. The samples were diluted with the Beltsville extender containing different concentrations of GPL according to the following groups: 0 (GPL-0), 0.1% (GPL-0.1), 0.5% (GPL-0.5), 1% (GPL-1), and 1.5% (GPL-1.5), then diluted semen was gradually cooled to 4°C during 3 hours and stored in liquid nitrogen. The optimum concentration of GPL was determined based on the quality parameters of thawed sperm. Our results showed sperm exposed to GPL-1 had significantly increased motion parameters and mitochondrial activity. The percentages of viability and membrane integrity were significantly higher in the GPL-1, and GPL-1.5 groups compared with the other groups (p < 0.05). Moreover, the lowest rate of apoptosis and lipid peroxidation were observed in the GPL-1 and GPL-1.5 groups in comparison with the frozen control group. Our findings indicated that membrane lipid replacement with GPL nanomicelles (1% and 1.5%) could substitute for damaged lipids in membranes and protect sperm cells against cryoinjury.

Mixed Mode Fracture Investigation of Rock Specimens Containing Sharp V-Notches.

This work aims to assess both experimentally and analytically the fracture behavior of rock specimens containing sharp V-notches (SV-notches) subjected to mixed mode I/II loading. To this end, firstly, several mixed mode fracture tests were conducted on Brazilian disk specimens weakened by an SV-notch (SVNBD sample), performed in their corresponding center and with various notch opening angles. Secondly, the fracture resistance of the tested samples was predicted using a criterion named MTS-FEM. This approach is based on the maximum tangential stress (MTS) criterion, in which the tangential stress is determined from the finite element method (FEM). Additionally, in the present research, the required critical distance is calculated directly from finite element analyses performed on cracked samples. Comparing the experimental results and the analytical predictions, it is shown that the fracture curves obtained from the MTS-FEM criterion are in agreement with the experimental results. These results are achieved without the need for the calculation of stress series expansion coefficients, as an additional advantage of the proposed approach.

Surgical treatment of a paraphimosis in a castrated alpaca secondary to a preputial avulsion.

A 4-year-old recently castrated male alpaca was referred for preputial prolapse of 4 d duration. Clinical findings upon admission were a 5 cm edematous preputial prolapse with the exposed preputial epithelium ulcerated and slightly necrotic. Initial therapy included NSAIDs and local treatment. A severe local inflammatory reaction with a consequent paraphimosis occurred on the penis and prepuce presumptively secondary to the use of a common compound ointment (i.e., tetracycline, scarlet oil, and lanolin) 24 h after its application. Medical treatment and local debridement were unsuccessful in reducing the paraphimosis. The prolapse was successfully reduced by traction of the penis through a para-preputial incision under general anesthesia. The alpaca was discharged after 22 d in hospital. The alpaca is still in the herd 24 mo after discharge with no prolapse recurrence.

Traitement chirurgical d'un paraphimosis chez un alpaga castré secondaire à une avulsion p réputiale. Un alpaga mâle de 4 ans récemment castré a été référé pour un prolapsus préputial d'une durée de 4 jours. Les signes cliniques à l'admission étaient un prolapsus préputial oedémateux de 5 cm avec l'épithélium préputial exposé ulcéré et légèrement nécrotique. Le traitement initial comprenait des AINS et un traitement local. Une réaction inflammatoire locale sévère avec un paraphimosis conséquent s'est produite sur le pénis et le prépuce, vraisemblablement secondaire à l'utilisation d'une pommade composée conventionnelle (i.e. tétracycline, huile scarlet et lanoline) 24 heures après son application. Le traitement médical et le débridement local n'ont pas réussi à réduire le paraphimosis. Le prolapsus a été réduit avec succès par traction du pénis à travers une incision parapréputiale sous anesthésie générale. L'alpaga est sorti après 22 jours d'hospitalisation. L'alpaga est toujours dans le troupeau 24 mois après sa sortie sans récidive de prolapsus.(Traduit par Dr Serge Messier).

Determination of Translaminar Notch Fracture Toughness for Laminated Composites Using Brazilian Disk Test.

This paper evaluates the fracture of notched epoxy matrix composites using the Brazilian disk (BD) test from both numerical and experimental points of view. The study began with a comprehensive experimental program covering three different composite lay-ups (quasi-isotropic, unidirectional, and cross-ply) and various geometries of U and V notches. Specifically, the BD samples combined the three layouts, four different notch angles, and three notch radii with three specimens per combination, leading to an overall number of 108 fracture tests. The experiments showed the appropriateness of the BD test for the study of the fracture behavior of composite materials and provided a good pool of data for further investigations. Subsequently, the virtual isotropic material concept (VIMC) was applied in combination with two fracture criteria to theoretically predict the experimentally acquired fracture loads. This study demonstrated that using the VIMC approach can provide robust predictions while incurring much lower computational costs compared to the conventional approaches found in the literature.

Tensile-Tearing Fracture Analysis of U-Notched Spruce Samples.

Spruce wood (Picea Mariana) is a highly orthotropic material whose fracture behavior in the presence of U-shaped notches and under combined tensile-tearing loading (so-called mixed-mode I/III loading) is analyzed in this work. Thus, several tests are carried out on U-notched samples with different notch tip radii (1 mm, 2 mm, and 4 mm) under various combinations of loading modes I and III (pure mode I, pure mode III, and three mixed-mode I/III loadings), from which both the experimental fracture loads and the fracture angles of the specimens are obtained. Because of the linear elastic behavior of the spruce wood, the point stress (PS) and mean stress (MS) methods, both being stress-based criteria, are used in combination with the Virtual Isotropic Material Concept (VIMC) for predicting the fracture loads and the fracture angles. By employing the VIMC, the spruce wood as an orthotropic material is modeled as a homogeneous and isotropic material with linear elastic behavior. The stress components required for calculating the experimental values of notch stress intensity factors are obtained by finite element (FE) analyses of the test configuration using commercial FE software from the fracture loads obtained experimentally. The discrepancies between the experimental and theoretical results of the critical notch stress intensity factors are obtained between -12.1% and -15% for the PS criterion and between -5.9% and -14.6% for the MS criterion, respectively. The discrepancies related to fracture initiation angle range from -1.0% to +12.1% for the PS criterion and from +1.5% to +12.2% for the MS criterion, respectively. Thus, both the PS and MS models have good accuracy when compared with the experimental data. It is also found that both failure criteria underestimate the fracture resistance of spruce wood under mixed-mode I/III loading.

A Modified Mean Stress Criterion for Considering Size Effects on Mode I Fracture Estimation of Rounded-Tip V-Notched Polymeric Specimens.

The aim of this paper is to assess the size and geometry effects on the mode I notch fracture toughness of polymeric samples containing rounded-tip V-shaped (RV) notches (V-notch with a finite radius at the notch tip). First, using a large number of fracture tests on an RV-notched Brazilian disk and semi-circular bending polymeric samples with four different sizes, the size-dependent values of the notch fracture toughness are obtained. Then, the mean stress criterion is modified for characterizing the size-dependency of notch fracture toughness in polymeric samples. The resulting modified mean stress criterion considers higher order terms of the stress field when calculating the fracture process zone length around the tip of the defect. Additionally, the critical distance rc is assumed to be associated with the specimen size and a formula containing fitting parameters is utilized for considering this trend of rc. The comparison between the values of notch fracture toughness obtained from experiments and those predicted by the modified mean stress criterion shows that the suggested approach can provide accurate estimations of size-dependent values of notch fracture toughness in polymeric specimens containing RV notches.

Impedance Variation and Learning Strategies in Human-Robot Interaction.

In this survey, various concepts and methodologies developed over the past two decades for varying and learning the impedance or admittance of robotic systems that physically interact with humans are explored. For this purpose, the assumptions and mathematical formulations for the online adjustment of impedance models and controllers for physical human-robot interaction (HRI) are categorized and compared. In this systematic review, studies on: 1) variation and 2) learning of appropriate impedance elements are taken into account. These strategies are classified and described in terms of their objectives, points of view (approaches), and signal requirements (including position, HRI force, and electromyography activity). Different methods involving linear/nonlinear analyses (e.g., optimal control design and nonlinear Lyapunov-based stability guarantee) and the Gaussian approximation algorithms (e.g., Gaussian mixture model-based and dynamic movement primitives-based strategies) are reviewed. Current challenges and research trends in physical HRI are finally discussed.

Combination of conserved recombinant proteins (NP & 3M2e) formulated with Alum protected BALB/c mice against influenza A/PR8/H1N1 virus challenge.

PURPOSE: Influenza is one of the most important agents of pandemic outbreak causing substantial morbidity and mortality. Vaccination strategies of influenza must be adapted annually due to constant antigenic changes in various strains. Therefore, the present study was conducted to evaluate protective immunity of the conserved influenza proteins. METHODS: For this purpose, three tandem repeats of M2e (3M2e) and NP were separately expressed in E. coli and were purified using column chromatography. Female Balb/c mice were injected intradermally with a combination of the purified 3M2e and NP alone or formulated with Alum (AlOH3) adjuvant in three doses. The mice were challenged by intranasal administration of H1N1 (A/PR/8/34) 2 weeks after the last vaccination. RESULTS: The results demonstrated that recombinant NP and M2e proteins are immunogenic and could efficiently elicit immune responses in mice compared to non-immunized mice. The combination of 3M2e and NP supplemented with Alum stimulated both NP and M2e-specific antibodies, which were higher than those stimulated by each single antigen plus Alum. In addition, the secretion of IFN-γ and IL-4 as well as the induction of lymphocyte proliferation in mice received the mixture of these proteins with Alum was considerably higher than other groups. Moreover, the highest survival rate (86%) with the least body weight change was observed in the mice immunized with 3M2e and NP supplemented with Alum followed by the mice received NP supplemented with Alum (71%). CONCLUSION: Accordingly, this regimen can be considered as an attractive candidate for global vaccination against influenza.

Applying nonlinear measures to the brain rhythms: an effective method for epilepsy diagnosis.

BACKGROUND: Epilepsy is a neurological disorder from which almost 50 million people have been suffering. These statistics indicate the importance of epilepsy diagnosis. Electroencephalogram (EEG) signals analysis is one of the most common methods for epilepsy characterization; hence, various strategies were applied to classify epileptic EEGs. METHODS: In this paper, four different nonlinear features such as Fractal dimensions including Higuchi method (HFD) and Katz method (KFD), Hurst exponent, and L-Z complexity measure were extracted from EEGs and their frequency sub-bands. The features were ranked later by implementing Relieff algorithm. The ranked features were applied sequentially to three different classifiers (MLPNN, Linear SVM, and RBF SVM). RESULTS: According to the dataset used for this study, there are five classification problems named ABCD/E, AB/CD/E, A/D/E, A/E, and D/E. In all cases, MLPNN was the most accurate classifier. Its performances for mentioned classification problems were 99.91%, 98.19%, 98.5%, 100% and 99.84%, respectively. CONCLUSION: The results demonstrate that KFD is the highest-ranking feature; In addition, beta and theta sub-bands are the most important frequency bands because, for all cases, the top features were KFDs extracted from beta and theta sub-bands. Moreover, high levels of accuracy have been obtained just by using these two features which reduce the complexity of the classification.

Critical Load Prediction in Notched E/Glass-Epoxy-Laminated Composites Using the Virtual Isotropic Material Concept Combined with the Average Strain Energy Density Criterion.

This paper attempts to validate the application of the Virtual Isotropic Material Concept (VIMC) in combination with the average strain energy density (ASED) criterion to predict the critical load in notched laminated composites. This methodology was applied to E/glass-epoxy-laminated composites containing U-notches. For this purpose, a series of fracture test data recently published in the literature on specimens with different notch tip radii, lay-up configurations, and a number of plies were employed. It was shown that the VIMC-ASED combined approach provided satisfactory predictions of the last-ply failure (LPF) loads (i.e., critical loads).

Application of chloroquine as an endosomal escape enhancing agent: new frontiers for an old drug.

INTRODUCTION: Adequate transfection efficiency is indispensable to safe and effective delivery of therapeutically active agents, particularly in cancer. Endosomal escape is regarded as a critical and determining step devoted a significant number of studies of the drug/gene delivery field. AREAS COVERED: This paper critically reviews the fundamental properties of chloroquine (CQ), its pharmacokinetics, pharmacodynamics, and clinical applications and the present knowledge of CQ application as an endosomal escape enhancing agent. Different approaches to enhance the endosomal escape process of nanoparticles have been introduced including use of endosomal escape enhancing agents. Application of CQ as either a pre-treatment modality in which cells or animals are exposed to CQ prior to the main treatment or a component of co-delivery systems where CQ and other anti-cancer agents are simultaneously entered the cancer cells, is discussed with recent studies. EXPERT OPINION: CQ is founded to intervene with the natural process of endosomal maturation. Moreover, CQ seems to increase the effectiveness of gene delivery by its electrostatic interaction with negatively charged components of the transferred genetic molecules. Endosomal escape might be regarded as the bottleneck of efficient gene delivery and CQ as an effective and available endosomal escape enhancing agent deserves more sophisticated studies.

Using robotic mechanical perturbations for enhanced balance assessment.

Balance impairment is critical for many patient groups such as those with neural and musculoskeletal disorders and also the elderly. Accurate and objective assessment of balance performance has led to the development of several indices based on the measurement of the center of pressure. In this study, a robotic device was designed and fabricated to provide controlled and repeatable mechanical perturbations to the standing platform of the user. The device uses servo-controlled actuators and two parallel mechanisms to provide independent rotations in mediolateral and anterior-posterior directions. The device also provides visual feedback of the center of pressure position to the user. Functional tests were run and showed that the device is able to provide an appropriate dynamics (time constant of 0.19 s and bandwidth of 0.85 Hz) for the two motions. The efficacy of the device on the balance assessment was then evaluated experimentally. Ten healthy subjects performed a balance task with and without perturbations and seven center of pressure indices were measured. It was shown that the sensitivity of the indices to the user's performance was statistically increased in all indices particularly in anterior/posterior direction when the mechanical perturbations were present.

Dynamic Reconfiguration of Redundant Haptic Interfaces for Rendering Soft and Hard Contacts.

There are conflicting objectives between required characteristics of haptic interfaces such as maximum force feedback capability versus back-drive friction, which can be optimally traded-off in a redundant haptic interface; a redundant haptic interface has more degrees of freedom than minimally required ones for a given task. In this article, a contact-aware null-space control approach for redundant haptic interfaces is proposed to address these trade-offs. First, we introduce a task-dependent null-space controller in which the internal motion of the redundant haptic interface is appropriately controlled to achieve a desired performance; i.e., low back-drive friction in case of free-space motion and soft contact or large force feedback capability in case of stiff contact. Next, a transition method is developed to facilitate the adaptation of the null-space controller's varying objectives according to the varying nature of the task. The transition method prevents discontinuities in the null-space control signal. This transition method is informed by a proposed actuator saturation observer that monitors the distance of joint torques from their saturation levels. The overall outcome is an ability to recreate the feelings of soft contacts and hard contacts with higher fidelity compared to what a conventional non-redundant haptic interface can achieve. Simulations are provided throughout the paper to illustrate the concepts. Moreover, experimental results are reported to verify the effectiveness of the proposed control strategies. It is shown that the proposed controller can perform well in the soft-contact, hard-contact, and transition phases.

Evaluation of the antitumor immune responses of probiotic Bifidobacterium bifidum in human papillomavirus-induced tumor model.

As of present, a number of studies have shown anti-cancer effects of different strains of probiotics, but the precise host immunological mechanisms of these antitumor effects remain unclear. Thus, the aim of current study was to investigate the preventive-therapeutic effects of oral versus intravenous administration of probiotic Bifidobacterium bifidum on immune response and tumor growth of C57BL/6 mice bearing transplanted TC-1 cell of human papillomavirus (HPV)-related tumor, expressing HPV-16 E6/E7 oncogenes. Our major findings are that the intravenous or oral administration of Bifidobacterium bifidum effectively induces antitumor immune responses and inhibits tumor growth in mice. Compared to oral route only, intravenous administration of probiotic Bifidobacterium bifidum into tumor-bearing mice leads to the activation of tumor-specific IL-12 and IFN-γ, lymphocyte proliferation, CD8+ cytolytic responses that control and eradicate tumor growth. These observations meant intravenous administration of probiotics is an effective anticancer approach through modulation of the immune system. The potential of probiotic Bifidobacterium bifidum as an immunomodulator in the treatment of cervical cancer could be further explored.

Granulosa cells exposed to fibroblast growth factor 8 and 18 reveal early onset of cell growth and survival.

BACKGROUND: Fibroblast growth factors (FGFs) are growth factors that have diverse biological activities including broad mitogenic and cell survival activities. They function through the activation of a specific tyrosine kinase receptor that transduces the signal by activating several intracellular signaling pathways. OBJECTIVE: To identify the different signaling pathways involved in the mechanism of action of FGF8 and FGF18 on ovine granulosa cells using mass spectrometry. MATERIALS AND METHODS: Ovine ovarian granulosa cells were harvested from adult sheep independently at the stage of the estrous cycle and were cultured at a density of 500,000 viable cells in 1 ml DMEM/F12 medium for five days. The cells were then treated on day 5 of culture with 10 ng/mL FGF8 and FGF18 for 30 minutes, and total cell protein was collected for mass spectrometry. RESULTS: Mass spectrometry showed that both FGF8 and FGF18 significantly induce simultaneous upregulation of several proteins, including ATF1, STAT3, MAPK1, MAPK3, MAPK14, PLCG1, PLCG2, PKCA, PIK3CA, RAF1, GAB1, and BAG2 ( > 1.5-fold; p < 0.01). CONCLUSION: ATF1 and STAT3 are important transcription factors involved in cell growth, proliferation and survival, and consequently can hamper or rescue the normal ovine reproductive system function.